Plants, Free Full-Text
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Last updated 20 setembro 2024
The Cattleya (Orchidaceae–Laeliinae subtribe) intergeneric hybrids, such as Brassolaeliocattleya (Blc.), have great ornamental value, due to their compact-size, with large and high color diversity of flowers. Artificial induction of polyploidy brings agronomic, ornamental and genetic benefits to plants. Polyploidization efficiency depends on factors, such as the type of antimitotic, polyploidization method, concentrations, exposure times and type of explant. This study aimed to develop a protocol to polyploidize Blc. orchids, by testing two types of explants (seeds and protocorms), concentrations and exposure times to colchicine. The effects of colchicine on the in vitro development of explants were also investigated. The responses of explants to colchicine depended on the concentrations, exposure time and the interaction of these factors. Flow cytometric analysis evidenced high endopolyploidy and allowed the separation of polyploidized (4C, 8C and 16C peaks) from non-polyploidized (only 2C and 4C peaks) plants. The highest percentage of polyploid plants was regenerated from protocorms (16.4%) treated with colchicine instead of seeds (3.2%). Protocorms treated with colchicine at 500–750 μM for 18 h resulted in the best percentage of polyploidization. Additionally, in vitro natural polyploidization using protocorms was reported (11.5%). Cytological analyses allowed an estimation of the number of chromosomes of the parents (≡70), polyploidized (≡140) and non-polyploidized progeny (≡70).
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Instant Guide to Medicinal Plants: Pamela Forey, Ruth Lindsay: 9780517691137: : Books
J Gen Plant Pathol (@JGenPlantPathol) / X
Insight into the root growth, soil quality, and assembly of the root-associated microbiome in the virus-free Chrysanthemum morifolium - ScienceDirect
This listing is for an instant download of clipart files to use on your next project!, You will be able to download the files immediately after
Plant Clipart House Plant Clipart Digital Download Plant Quotes Hand Drawn Plants, Plant Lady Plant Mom Plant Lover Instant Download Clipart
Frontiers Identifying Effective Design Approaches to Allocate Genotypes in Two-Phase Designs: A Case Study in Pelargonium zonale
FREE Plant Life Cycle Worksheets and Activity Pack
Sttm Series Get File - Colaboratory
Garden State Bulb Raspberry Heritage 1 Year Live Bare Root Plant (Bag of 1) ECS-15-01-01 - The Home Depot
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